HPLC Chiral Analysis and Stereo-Bioactivity of Repaglinide Enantiomers
Keywords:
Repaglinide, HPLC, Chiralcel®OD-RH, molecular docking, antidiabetic, chiral separationAbstract
In the present study, a simple, precise and rapid chiral High performance Liquid Chromatography (HPLC) method has been developed and validated for the enantiomeric separation of Repaglinide, with the molecular docking study of Repaglinide against carbonic anhydrase II (CA2). The method was carried out on a chiralcel® OD-RH column using a mixture of n-hexane: 2-propanol (95:5, v/v), The flow rate was 1.0 ml/min and the eluent was monitored at 240nm. The software used in this molecular docking is PyRx-Virtual Screening Tools (for molecular docking process) and Discovery Studio (for pose visualization and data analysis). According to the study's findings, for HPLC two peaks with a 2.074- and 3,258-min retention time was achieved and the resolution, capacity and selectivity factors obtained were Rs = 0.773; k′1= 1.074, k′2 = 2.258 respectively and α =2,1. The result of molecular docking showed the binding energy of repaglinide to CA2 was found to be -6.8 kcal/mol with RMSD of 1.55 Å, indicating a higher binding affinity compared to native ligand (dansylamide).The method was found to be fast, simple, precise and suitable for analysis of Repaglinide in drug substance.
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